食品科学2018，Vol.39Issue(4)：225-232,8.DOI:10.7506/spkx1002-6630-201804034
应用体外全仿生模型初步分析2种富硒产品中硒形态及生物可给性
Speciation Analysis and Bioavailability Assessment of Selenium in Two Selenium-Enriched Foods by in Vitro Biomimetic Gastrointestinal Tract Models
摘要
Abstract
An effective method was developed for the speciation analysis of selenium in digests of Se-enriched Cordyceps militaris and selenium-enriched yeast prepared using in vitro biomimetic gastrointestinal tract models by microwave digestion-high resolution-continuum source graphite furnace atomic absorption spectrophotometry (HR-CS GFAAS). The aim was to study the influences of gastric and intestinal digestion on the speciation and bioavailability of Se in selenium-enriched food. The gastric and intestinal digests were separated through a 0.45 μm membrane filter into suspended and soluble Se, and inorganic and organic Se from the soluble fraction were further separated using D101 macroporous resin. Moreover, Se-binding protein and polysaccharide were precipitated by acetone and ethanol, respectively. The partition behaviors of Se from Se-enriched food in the stomach and gut were simulated with n-octyl alcohol and monolayer liposome as cell membrane, respectively. The optimal analysis wavelength for Se was 196.026 7 nm. A mixture of 1 g/L Pd(NO3)2and 0.5 g/L Mg(NO3)2(5 μL) was selected as the best matrix modifier, and an ashing temperature of 1 100 ℃ and an atomization temperature of 2 200 ℃ were used.The results showed that Se contents in selenium-enriched Cordyceps militaris and selenium-enriched yeast were determined to be 2.129 and 27.75 μg/g, respectively under these conditions. The contents of all Se forms except suspended Se in the intestinal digest were higher than in the gastric digest. n-Octyl alcohol-soluble Se and monolayer liposome-bound Se were more abundant than water-soluble Se during simulated intestinal digestion.关键词
体外全仿生模型/富硒/形态分析/生物可给性/高分辨-连续光源石墨炉原子吸收光谱法Key words
in vitro biomimetic gastrointestinal tract models/selenium-enriched/speciation analysis/bioavailability assessment/high resolution-continuum source graphite furnace atomic absorption spectrophotometry (HR-CS GFAAS)分类
化学化工引用本文复制引用
陈尚龙,刘恩岐,陈安徽,刘辉,巫永华,秦旭..应用体外全仿生模型初步分析2种富硒产品中硒形态及生物可给性[J].食品科学,2018,39(4):225-232,8.基金项目
江苏省高等学校自然科学研究项目(17KJB550007) （17KJB550007）
江苏省前瞻性联合研究项目(BY2015024-04) （BY2015024-04）
江苏省苏北科技专项(BN2106017) （BN2106017）
国家自然科学基金面上项目(31270577) （31270577）
