食品科学2018，Vol.39Issue(4)：312-319,8.DOI:10.7506/spkx1002-6630-201804047
转基因大豆MON89788双重数字PCR通用定量检测方法的建立
An Universal Quantitative Detection Method for Genetically Modified Soybean Event MON89788 Using Duplex Digital PCR
摘要
Abstract
An universal quantitative detection method for genetically modified (GM) soybean event MON89788 using duplex digital PCR (dPCR) with satisfying specificity, stability and sensitivity was established in this paper. This method could quantify both the endogenous and exogenous genes in a single reaction system and was demonstrated to be suitable for both droplet digital PCR (ddPCR) and chip digital PCR (cdPCR) platforms. The method then was used to qualify blind samples by a food analysis performance assessment scheme (FAPAS) test. The absolute limits of quantitation (LOQs) of ddPCR for MON89788 event-specific gene and lectin endogenous gene were 8.0 copies/μL and 8.2 copies/μL, respectively, while those of cdPCR were 7.443 copies/μL and 7.646 copies/μL, respectively. The relative LOQs of MON89788 were both 0.1% by ddPCR and cdPCR.关键词
微滴式数字PCR/芯片式数字PCR/转基因大豆MON89788品系/定量检测Key words
droplet digital PCR/chip digital PCR/GM soybean event MON89788/quantitative detection分类
生物科学引用本文复制引用
刘津,李婷,冼钰茵,吴希阳,凌莉,高东微..转基因大豆MON89788双重数字PCR通用定量检测方法的建立[J].食品科学,2018,39(4):312-319,8.基金项目
广东省科技计划项目(2014A040401029 （）
2015A030401042) （）
国家质检总局科技计划项目(2016IK055) （2016IK055）
出入境检验检疫行业标准制定计划项目(2015B218k) （2015B218k）
