食品科学2018，Vol.39Issue(4)：320-324,5.DOI:10.7506/spkx1002-6630-201804048
基于ATP再生体系快速检测乳品中微生物
Development of a Bioluminescence Method Combined with ATP Amplification for Detection of Bacteria in Dairy Products
摘要
Abstract
A bioluminescence method combined with adenosine triphosphate (ATP) amplification through regeneration from pyrophosphate (PPi) was developed to detect bacteria in dairy products. Experimental conditions were optimized by one-factor-at-a-time method. The sensitivity, precision and accuracy of this method were studied. The results showed that adenosine phosphosulfate (APS) concentration of 10 μmol/L, ATP sulfurylase (ATPS) activity of 0.15 U/mL and pH of 7.8 were found to be the optimum conditions. The method showed a good linearity within the range of 102–107CFU/mL with lowest limits of detection (LODs) of 10?17mol/mL, 102CFU/mL and 102CFU/mL for ATP, Escherichia coli and Pseudomonas aeruginosa, respectively. The recoveries of E. coli in dairy at three spiked levels ranged from 81.33% to 97.78%, with coefficients of variation between 14.24% and 22.17%. Good correlation between this method and the standard plate count method was obtained using spiked dairy samples. The proposed method is fast, simple, sensitive, stable, and thus suited for fast detection of bacteria in dairy products.关键词
ATP再生/生物发光/快速检测/乳品Key words
ATP amplification/bioluminescence assay/fast detection/dairy products分类
轻工纺织引用本文复制引用
常超,王凌,伍金娥..基于ATP再生体系快速检测乳品中微生物[J].食品科学,2018,39(4):320-324,5.基金项目
湖北省教育厅科学技术研究重点项目(D20161701) （D20161701）
