食品与发酵工业2018，Vol.44Issue(1)：217-223,7.DOI:10.13995/j.cnki.11-1802/ts.015664
基于反转录-环介导等温扩增技术检测金黄色葡萄球菌
Staphylococcus aureus detection by reverse transcription loop-mediated isothermal amplification
梁玉林 1刘秀 1周振森 1周鹏飞 1尹建军1
作者信息
- 1. 中国食品发酵工业研究院,北京,100015
- 折叠
摘要
Abstract
In this paper,a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) method has been developed to detect Staphylococcus aureus.Series of primers were designed according to the specific conservative nuc gene,The optimized primer usage and reaction conditions for Staphylococcus aureus detection using RT-LAMP was established.The specificity and sensitivity of the method was studied using Staphylococcus aureus-contaminated skim milk,and was compared to the reverse transcription polymerase chain (RT-PCR) method in terms of sensitivity.The RT-LAMP assay successfully detected Staphylococcus aureus within 30 minutes at 65 ℃.There were 5 out of 26 strains of Staphylococcus aureus detected.The sensitivity of detecting the nucleotide extract of the contaminated skim milk samples was 230 CFU/g,which was 100 fold higher than that of RT-PCR.This RT-LAMP method is a powerful way for rapid detection for Staphylococcus aureus.关键词
环介导/金黄色葡萄球菌/靶基因/反转录/活菌Key words
loop mediated/Staphylococcus aureus/target gene/reverse transcription/viable bacteria引用本文复制引用
梁玉林,刘秀,周振森,周鹏飞,尹建军..基于反转录-环介导等温扩增技术检测金黄色葡萄球菌[J].食品与发酵工业,2018,44(1):217-223,7.
