食品与机械2017，Vol.33Issue(11)：23-27,5.
烟叶蛋白高效降解菌的筛选鉴定及其作用效果研究
The study on isolation, screening, identification and application effect of an efficient protein derading bacteria in flue-cured tobacco
摘要
Abstract
In order to degrade protein of tobacco and improve the smoking quality of tobacco,18 protein degrading bacterias from flue cured tobacco of Henan,Fujian,Yunnan province were isolatedthrough protein degrading selective medium.The activity of protease was detected,and the strain with highest protease-producing was identified by the 16S rRNA gene sequence and physiological-biochemical tests.Orthogonal experiment was further designed to get the optimal fermentation conditions of protein degrading in the tobacco leaf.And then the sensory quality of tobacco leaves after treatment was investigated The results showed that:(1) Strain HN-3 was the preferred strain with the highest activity of protease enzyme producing (3 417 U/mL).(2) Strain HN 3 was identified as Bacillus pumilus by the 16S rRNA gene sequence and physiological biochemical tests.(3) The orthogonal experiment results suggested that,by adding 3％ culture medium (with strain HN-3) to the surface of tobacco leaf,the degrading rates of protein could reach 29.66％ after 84 hours fermentation under the 37 ℃.(4) The sensory evaluation results showed that after fermentation,the aroma of tobacco leaves was changed well.The amount of aroma and smoke increased,while the gas content decreased,the aftertaste improved,and the overall sen sory quality of tobacco was improved obviously.关键词
烟叶/蛋白质/短小芽孢杆菌/蛋白酶/发酵Key words
tobacco/protein/Bacillus pumilus/protease/fermentation引用本文复制引用
杨宗灿,王红霞,冯颖杰,刘超,王鹏飞,张展,张耀广,杨永锋,李家美,刘向真..烟叶蛋白高效降解菌的筛选鉴定及其作用效果研究[J].食品与机械,2017,33(11):23-27,5.基金项目
河南中烟科技创新项目(编号:ZW2014035) （编号:ZW2014035）
