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九头狮子草抗菌有效组分分析

张丽娟 王正 廖尚高

食品与机械2017,Vol.33Issue(11):38-40,46,4.
食品与机械2017,Vol.33Issue(11):38-40,46,4.

九头狮子草抗菌有效组分分析

Antibacterial effective component analysis of Peristrophe japonica

张丽娟 1王正 2廖尚高3

作者信息

  • 1. 贵州理工学院食品药品制造工程学院,贵州贵阳550001
  • 2. 湖北医药学院药学院,湖北十堰442000
  • 3. 贵州省药物制剂重点实验室,贵州贵阳550004
  • 折叠

摘要

Abstract

To determine the antibacterially effective fraction from Peristrophe japonica (Thunb.) Brem.and to clarify its chemical components.The ethanol extract of Peristrophe japonica (Thunb.) Brem.was separated by macroporous resin column chromatography eluted with water,80% ethanol and acetone,respectively,to give the fractions A C.The in vitro antibacterial effects of the fractions were determined by the disc diffusion test.The anti-bacterial potencies of the active fraction was then assessed in vitro by determining the MICs and MBCs.The chemical structures and relative contents of the components present in the antibacterially effective fraction were determined by gas chromatography mass spectrometry (GC-MS).Each fraction from Peristrophe japonica (Thunb.) B rem showed different ievels of antibacterial activity with fraction C (the acetone fraction) being the most active fraction.Twenty six com pounds were identified by GC-MS in the effective fraction,these compounds are fatty acid and its derivatives.The fatty acid and its derivatives is the main antibacterially active ingredients in Peristrophe japonica.

关键词

九头狮子草/抗菌活性/有效组分分析/GC-MS

Key words

Peristrophe japonica/antibacterial activity/effective component analysis/GC-MS

引用本文复制引用

张丽娟,王正,廖尚高..九头狮子草抗菌有效组分分析[J].食品与机械,2017,33(11):38-40,46,4.

基金项目

贵州省大学生创业基金(编号:T2010-22) (编号:T2010-22)

贵州省科技厅贵州省优秀青年科技人才项目(编号:[2015]32号) (编号:[2015]32号)

贵州省科学技术基金(编号:黔科合基础[2017]1075) (编号:黔科合基础[2017]1075)

食品与机械

OA北大核心CSCDCSTPCD

1003-5788

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