食品与机械2017，Vol.33Issue(11)：57-60,4.
基于纳米金复合探针的沙门氏菌快速定量检测
Rapid quantitative detection of Salmonella based on nanogold composite probes
摘要
Abstract
As the defects of the existing detection methods for Salmonella,a fast,simple,low cost and highly sensitive new rapid detection technology has been developed.The CdTe quantum dots were synthesized by water method,and then the display DNA was used to connect hundreds of quantum dots to a gold nanoparticle.After preparing the amination Fe3 O4 magnetic nanoparticles by using a water hot-solvent hot method,the principle of avidin-biotin specific was used to bind with the capturing probes.Finally the fluorescence in tensity of Salmonella DNA was detected.The results showed thatthe fluorescence intensity and Salmonella DNA concentration had good linear relationship in the range of 10 ～ 1 000 fmol/L,and the regression equation was I F =0.198 [DNA] + 55.00,R 2 =0.997,with the detection limit of 8 fmol/L.Moreover,it also showed good accuracy,with the minimum detection limit of 4 fmol/L,in the detection of milk samples contaminated with salmonella.关键词
CdTe量子点/纳米金/磁性纳米粒子/沙门氏菌Key words
CdTe quantum dots/gold nanoparticle/magnetic nanoparticles/Salmonella引用本文复制引用
谢同彬,梅林..基于纳米金复合探针的沙门氏菌快速定量检测[J].食品与机械,2017,33(11):57-60,4.基金项目
安徽省2017年面上科技攻关项目(编号:1704g07020104) （编号:1704g07020104）
