食品与机械2017，Vol.33Issue(11)：67-71,5.
全自动凯氏定氮仪测定大米蛋白质
Study on determination of rice protein by automatic kjeldahl apparatus
郭应时 1曹小彦 1邹杭君 1郭明义 2张威1
作者信息
- 1. 广电计量检测[湖南]有限公司,湖南长沙410007
- 2. 长春市食品药品监督管理局,吉林长春130000
- 折叠
摘要
Abstract
The method of measuring rice protein with fully automatic Kieldahl azotometer by single-factor experiment and orthogonal experiment was investigated.It defines the best experiment conditions through examining such key factors as sample weight,additive a mount of sulfuric acid,digesting temperature and digesting time.The results showed that when the sample weight is 0.40 g,the added amount of sulfuric acid was 12 mL,digested at is 420 ℃ and for 50 min,the experimental effect was the best.The standard deviations of 6 determinations of 5 kinds of rice were 0.050 2～0.101 1,and the recoveries of nitrogen of urea were 99.9％ ～ 100.5％.The ratios of protein obtained by semi-micro distillation method were between 1.023 4～1.079 4,and the time of determination could be shortened to 1 hours.The method was found stable and accurate.关键词
蛋白质/全自动凯氏定氮仪/样品质量/硫酸添加量/消化温度/消化时间Key words
protein/automatic kjeldahl apparatus/sample weight/concentrated sulfuric acid/digestion temperature/digestion time引用本文复制引用
郭应时,曹小彦,邹杭君,郭明义,张威..全自动凯氏定氮仪测定大米蛋白质[J].食品与机械,2017,33(11):67-71,5.
