食品工业科技2018，Vol.39Issue(3)：32-38,7.DOI:10.13386/j.issn1002-0306.2018.03.007
双孢菇蛋白粉废料中多糖的分离纯化及抗氧化活性研究
Isolation, purification and antioxidant activity of polysaccharide from Agaricus bisporus protein powder waste
摘要
Abstract
Objective:The polysaccharide fractions and antioxidant activities of Agaricus bisporus protein powder waste were analyzed.Method:The Agaricus bisporus fruitbody protein extracted after residue was used as material.Agaricus bisporus crude polysaccharide was prepared using ultrasonic hot water extractive method,Sevage method,D101 macroporous resin and alcohol precipitation.The purified polysaccharide was obtained by DEAE-52 chromatography column.By scanning electron microscopy (SEM),infrared spectroscopy(IR) method for identification of physical and chemical properties of the purified polysaccharide.The antioxidant activity of the purified polysaccharide was evaluated by scavenging DPPH free radical,superoxide anion free radical and ABTS free radical,and protecting the yeast cells treated with H2O2.The activities of T-SOD,MDA and GSH-Px in the supernatants of H2 O2 oxidative damage were detected.Results:Three fractions (ABPS-Ⅰ,ABPS-Ⅱ and ABPS-Ⅲ) were isolated and purified from the crude polysaccharide of Agaricus bisporus.Studies showed that ABPS-Ⅲ had the strongest scavenging capacity for DPPH radicals.The content of polysaccharide from ABPS-Ⅲ was 89.12％,and SEM had hexahedron structure,and IR analysis showed that it had obvious characteristics of carbohydrate compounds.The EC50 value of scavenging DPPH,superoxide anion and ABTS radical of ABPS-Ⅲ was 1.48,1.85,1.30 mg/mL,respectively.Oxidative damage to yeast cell protection experiments showed that ABPS-Ⅲ could significantly improve the survival rate of injury of yeast cell oxidation,at the concentration of 25 mg/mL,the protection rate of 42.58％.Enzyme activity assay showed that after adding ABPS-Ⅲ,compared with the oxidized injury group,the activity of T-SOD and GSH-Px in the supernatant of yeast cells increased by 337％ and 102％ respectively(p ＜ 0.01),and the content of MDA decreased by 35.17％ (p ＜ 0.05).Conclusion:Agaricus bisporus polysaccharide ABPS-Ⅲ has strong antioxidant activity.关键词
双孢菇多糖/抗氧化活性/酵母细胞/氧化损伤Key words
Agaricus bisporus polysaccharide/antioxidant activity/yeast cells/oxidative damage分类
轻工纺织引用本文复制引用
胡亚平,张金秀,赵立强,鲁玉佳,王立安..双孢菇蛋白粉废料中多糖的分离纯化及抗氧化活性研究[J].食品工业科技,2018,39(3):32-38,7.基金项目
河北省科技计划重点项目(16237301D) （16237301D）
河北省现代农业产业技术体系创新团队项目(HBCT2013060201) （HBCT2013060201）
河北省高等学校科学技术研究项目(ZC2016059) （ZC2016059）
邢台市科技计划项目(2015ZC167). （2015ZC167）
