食品工业科技2018，Vol.39Issue(3)：97-100,4.DOI:10.13386/j.issn1002-0306.2018.03.020
紫外-硫酸二乙酯复合诱变高通量筛选虫草素高产蛹虫草突变株
High-throughput screening of Cordyceps militaris for cordycepin production by UV-DES compound mutation
摘要
Abstract
In order to further improve the cordycepin yield,Cordyceps militaris(C.militaris) CICC 14014 was considered as the starting strain for protoplast compound mutation.The protoplasts of the strain were induced by ultraviolet-diethyl sulfate(UV-DES).Then,a high cordycepin yield and stable genetic strain was obtained by screening with 96-well microplates and triangle bottles.Through primary.screening with 96-well microplates,54 mutant strains with a yield greater than 6 g/L cordycepin were obtained.A strain of cordycepin high yield mutant strain UD10-2 was screened out by secondary screenings with triangle bottles.The cordycepin production obtained by C.militaris UD10-2 mutant was 11.32 g/L in liquid surface culture,which was enhanced by 108.47％ more than the original strain(5.43 g/L).The results of subculture test showed that the mutant strain had stable hereditary characteristics after 20 generations.关键词
复合诱变/蛹虫草/液体表面培养/高通量筛选/虫草素Key words
compound mutation/Cordyceps militaris/liquid surface culture/high-throughput screening/cordycepin分类
轻工纺织引用本文复制引用
汤佳鹏,汪建雄..紫外-硫酸二乙酯复合诱变高通量筛选虫草素高产蛹虫草突变株[J].食品工业科技,2018,39(3):97-100,4.基金项目
江苏省普通高校自然科学研究计划项目(16KJB530004) （16KJB530004）
南通市应用基础研究项目(MS12015066). （MS12015066）
