食品工业科技2018，Vol.39Issue(3)：117-121,126,6.DOI:10.13386/j.issn1002-0306.2018.03.024
醋酸菌发酵产细菌纤维素培养基和培养条件的优化
Optimization of fermentation medium and culture conditions of producing bacterial cellulose of Gluconacetobacter hensennii strain
陈海超 1王延斌 1熊强1
作者信息
- 1. 南京工业大学食品与轻工学院,江苏南京211800
- 折叠
摘要
Abstract
Single factors tests combined response surface design were carried out to optimize fermentation medium of producing bacterial cellulose of Gluconacetobacter hensennii strain.The strain was isolated by our laboratory from traditional kombucha.Besides,based on the optimized medium,culture conditions were optimized by single factors tests and orthogonal experiments.The results showed that the optimal medium(g/L) contained 10 g sucrose,9.36 g yeast extract powder,5 g peptone,2.86 g disodium hydrogen phosphate,0.3 g citric acid monohydrate,pHS.96.Under the condition,BC dry weight was 4.779 g/L.Based on the medium,performed fermenting progress in 50 mL medium with 10％ seed solution at 30 ℃ for 10 d.Under the condition,BC dry weights could arrived 8.515 g/L and the growth rate was more than 300％ compared to control group.关键词
汉逊氏葡糖酸醋杆菌/细菌纤维素/发酵培养基/响应面设计/正交实验/优化Key words
Gluconacetobacter hensennii/bacterial cellulose/fermentation medium/response surface design/orthogonal experiment/optimization分类
轻工纺织引用本文复制引用
陈海超,王延斌,熊强..醋酸菌发酵产细菌纤维素培养基和培养条件的优化[J].食品工业科技,2018,39(3):117-121,126,6.
