食品工业科技2018，Vol.39Issue(3)：158-166,9.DOI:10.13386/j.issn1002-0306.2018.03.032
红曲废菌渣中有效成分的综合提取工艺
The integrated extraction process of effective ingredients from monascus mycelium
摘要
Abstract
On the basis of single factor experiment,the extraction conditions of monascus pigment and crude protein,the saponification conditions of ergosterol and the deacetylation conditions of chitosan were optimized by orthogonal test in order to improve the utilization rate of the active ingredients of the mycelium.The results showed:the optimum extraction condition of monascus pigment was ethanol concentration 80％,temperature 50 ℃,solid-liquid ratio 1∶ 34 (g∶ mL),and extraction time 30 min;the optimum extraction condition of protein was pH9,temperature 45 ℃,time 2 h;the optimum saponification condition of ergosterol was NaOH concentration 14％,temperature 90 ℃,time 2 h,and solid-liquid ratio 1 ∶ 8 (g∶ mL);the optimum deacetylation condition of chitosan was alkali concentration45％,temperature 110 ℃,time 3 h.In the end,the recoveries of red pigment,crude protein,ergosterol and chitosan were up to 94.41％,65.23％,4.16％ and 3.64％,respectively.This approach can be used to extract monascus pigments,crude protein,ergosterol and chitosan at one time to achieve maximum utilization of resources.关键词
红曲霉菌丝体/红曲色素/粗蛋白/麦角固醇/壳聚糖/提取Key words
monascus mycelium/monascus pigment/protein/ergosterol/chitosan/extraction分类
轻工纺织引用本文复制引用
董晔,杨姿,贾梦琦,耿子茵,牛启启,杨雪莲..红曲废菌渣中有效成分的综合提取工艺[J].食品工业科技,2018,39(3):158-166,9.基金项目
“十三五”国家重点研发计划项目课题2016YFD0400802“食用色素绿色制造技术研究” （）
北京市级大学生科学研究与创业行动计划201710011101. （）
