食品工业科技2018，Vol.39Issue(3)：209-214,6.DOI:10.13386/j.issn1002-0306.2018.03.041
草鱼边角料蛋白质水解工艺
Enzymatic hydrolysis process research of grass carp offal
摘要
Abstract
In this study,the complex of trypsin and flavor protease was used to deeply hydrolyze grass offal.Response surface methodology(RSM)was applied to optimize the enzymatic hydrolysis process of grass offal protein and maximize the content of free amino acids in its hydrolysates by assessing the factors of enzymatic hydrolysis temperature,pH,liquid material ratio and enzyme addition,using total free amino acids as response factor.The result showed that the complex of trypsin and flavor protease had an effective hydrolysis on grass carp.The maximum ∑ FAA of its hydrolysates could be obtained when composite ratio was 3∶ 1.In addition,temperature,pH,solid-liquid ratio and enzyme dosage had a significant (p ＜ 0.05)effect on its hydrolysis degree.The maximum ∑ FAA of its hydrolysates was (12.03 ± 0.11) mg/mL when temperature was 50 ℃,pH was 7.3,liquids to material ratio was 4.2∶1,enzyme dosage was 2.2％ and time was 6 h.17 kinds of free amino acids were detected in the enzymatic hydrolysate.The content of different amino acids increased significandy as the time of enzymatic hydrolysis was extended.When the time of hydrolysis was over 6 h,increased trend of free amino acids obviously slow down.Hence,the established preparation technology could be used to produce protein hydrolysates of grass offal.关键词
草鱼/酶解/游离氨基酸/响应曲面Key words
grass carp/enzymatic hydrolysis/free amino acid/response surface分类
轻工纺织引用本文复制引用
谢广杰,闫静芳,颜智勇,王昱沣..草鱼边角料蛋白质水解工艺[J].食品工业科技,2018,39(3):209-214,6.基金项目
江苏省科技厅苏北科技专项(BN2015071)及企业横向课题(鱼类下脚料生物法制备新型猫粮诱食剂的研究). （BN2015071）
