食品工业科技2018，Vol.39Issue(3)：215-219,225,6.DOI:10.13386/j.issn1002-0306.2018.03.042
铁皮石斛多糖复合酶法提取工艺及其抗氧化性
Extraction process of polysaccharide by compound enzymatic method of Dendrobium officinale and its antioxidant activity
薛燕 1敢小双 2黄开丽 1李丽 1王巧娥 1董银卯1
作者信息
- 1. 中国轻工业化妆品重点实验室,北京工商大学,北京100048
- 2. 无限极(中国)有限公司,广东广州510623
- 折叠
摘要
Abstract
In this paper,the suitable enzymatic extraction method and the antioxidant activity of Dendrobium officinale polysaccharides(DOP)were studied.The effects of enzymatic ratio,enzyme concentration,temperature,extraction time,solidliquid ratio and pH on yield of polysaccharides were investigated,on the basis of single-factor experiments and orthogonal test for enzymatic action.The optimized extraction conditions were:Dispase and Cellulase ratio of 2∶ 1,enzyme concentration of 10％,solid-liquid ratio of 1∶ 120,extraction temperature of 55 ℃,pH6.0,extraction time of 3 h,the yield of polysaccharides were 43.85％ ± 1.8％,antioxidation results showed that the DOP extracted by enzymatic method had an obvious antioxidant activity,the IC50 values of DPPH and ABTS free radical scavenging abilities were 1.331,0.467 mg/mL,Dendrobium officinale polysaccharides had good antioxidant activity.关键词
铁皮石斛/多糖/复合酶法/抗氧化活性Key words
Dendrobium officinale/polysaccharides/enzymatic action/antioxidant activity分类
轻工纺织引用本文复制引用
薛燕,敢小双,黄开丽,李丽,王巧娥,董银卯..铁皮石斛多糖复合酶法提取工艺及其抗氧化性[J].食品工业科技,2018,39(3):215-219,225,6.
