食品工业科技2018，Vol.39Issue(3)：305-310,6.DOI:10.13386/j.issn1002-0306.2018.03.058
紫薯花色苷对小鼠淋巴细胞免疫功能的调节作用
Regulatory effects of purple sweet potato anthocyanins on lymphocyte immune function in mice
摘要
Abstract
To investigate the regulatory effects of purple sweet potato anthocyanins (PSPA),preparation of mice splenic lymphocytes,the effect of PSPA on lymphocyte was detected with MTT assay.The cell membrane surface marker and cell cycle of PSPA on splenic lymphocyte was detected by flow cytometry.The results showed that lymphocyte stimulation index of all concentration PSPA group (p ＜ 0.01)was extremely significantly higher than the blank control group (p ＜ 0.01),the lymphocyte stimulation index of 20 mg/mL PSPA group was extremely significantly higher than positive control group (p ＜ 0.01).The CD4+/CD8 + ratio of lymphocyte subsets of 2.5 mg/mL PSPA group was significantly higher than blank control group(p ＜ 0.05),the CD4 +/CD8 + ratio of lymphocyte subsets of 5,10 and 20 mg/mL PSPA group was extremely significantly higher than blank control group (p ＜ 0.01).The CD4+ CD8 + percentage of lymphocyte subsets was extremely significantly higher 2.0 mg/mL PSPA group than positive control group (p ＜ 0.01).The percentage of lymphocytes in the S phase of all concentration PSPA group was extremely significantly higher than the blank control group (p ＜ 0.01).The percentage of lymphocytes in the S phase of 20 mg/mL concentration PSPA group was extremely significantly higher than positive control group(p ＜0.01).It was Indicated that PSPA can promoted lymphocyte proliferation,increased CD4 +/CD8 + subgroup ratio,increased the percentage of cells in the S phase,thereby regulated the immune function of lymphocytes.Conclusion:PSPA has obvious immunomodulatory activity.关键词
紫薯花色苷/淋巴细胞/CD4 +/CD8+/细胞周期Key words
purple sweet potato anthocyanins/lymphocytes/CD4+/CD8+/cell cycle分类
轻工纺织引用本文复制引用
陈焱坤,刘晓玲,李敬双,王会,于洋..紫薯花色苷对小鼠淋巴细胞免疫功能的调节作用[J].食品工业科技,2018,39(3):305-310,6.基金项目
辽宁省自然科学基金(20170540357) （20170540357）
2017年国家级“大学生创新创业训练计划”项目(201610160055). （201610160055）
