食品工业科技2018，Vol.39Issue(3)：330-332,337,4.DOI:10.13386/j.issn1002-0306.2018.03.063
多酚氧化酶的提取及分离纯化研究进展
Progress in extraction and purification of polyphenol oxidase
摘要
Abstract
Polyphenol oxidase(PPO) is a widely distributed in the nature of a metalloproteinase,it can catalyze the oxidation of o-benzenediol to o-benzodioxanone.And it is also the main reason for enzymatic browning of many fruits,vegetables and other agricultural products.In this paper,the recent advances in the extraction and purification of polyphenol oxidase from domestic and foreign were reviewed,including traditional extraction methods(such as buffer homogenization,acetone extraction,acetone powder extraction,etc.),new extraction methods (ultrasonic-assisted extraction and ultra-high pressure extraction,etc.),and also including the traditional classical separation and purification methods(such as solvent method,precipitation method),the new separation and purification methods(such as gel chromatography and ion exchange chromatography,etc.).The aim of this study is to provide reference for the research and application of polyphenol oxidase,especially for the inhibition of enzymatic browning in agricultural products.关键词
多酚氧化酶/提取/分离纯化Key words
polyphenol oxidase/extraction/separation and purification分类
轻工纺织引用本文复制引用
陈桂,李维,张海洲,张莉,梁春华..多酚氧化酶的提取及分离纯化研究进展[J].食品工业科技,2018,39(3):330-332,337,4.基金项目
湖南省科技厅重点研发项目(2016NK2134) （2016NK2134）
湖南省教育厅科研项目基金(17C1261) （17C1261）
怀化学院科研项目(HHUY2017-07). （HHUY2017-07）
