食品工业科技2018，Vol.39Issue(4)：143-149,7.DOI:10.13386/j.issn1002-0306.2018.04.027
响应面法优化东北红松针总黄酮的超声辅助乙醇提取工艺
Optimization of ultrasonic-assisted ethanol extraction of total flavonoids from Pinus koraiensis needles by response surface methodology
摘要
Abstract
The aim of this paper was to extract the total flavonoids from Xiaoxing'anling Pinus koraiensis needles by ultrasonic assisted ethanol extraction method,and to achieve the purpose of rational utilization of the Northeast Pinus koraiensis needles.The ratio of material to liquid,volume fraction of ethanol,ultrasonic temperature,ultrasonic time were set as the main influencing factors,based on the single factor experiment,the total flavonoid yield was taken as the object of study,BoxBehnken center combination design and response surface methodology were used to optimize the extraction process of total flavonoids from Pinus koraiensis needles.The results showed that when the ratio of material to liquid was 1 ∶ 52 g/mL,the volume fraction of ethanol was 51％,the ultrasonic temperature was 76 ℃ and the time was 103 min,the yield of total flavonoids was about 5.82％.The process conditions are reasonable and reliable,which provides a theoretical basis for industrial production and extraction.关键词
红松/总黄酮/超声提取/响应面法Key words
Pinus koraiensis/total flavonoids/ultrasonic extraction/response surface methodology分类
轻工纺织引用本文复制引用
王冉,李健,黎晨晨,刘宁,刘涛..响应面法优化东北红松针总黄酮的超声辅助乙醇提取工艺[J].食品工业科技,2018,39(4):143-149,7.基金项目
黑龙江省应用技术研究与开发计划项目(GC13C112) （GC13C112）
黑龙江省教育厅科学技术面上项目(12541199) （12541199）
2016年哈尔滨商业大学青年创新人才支持项目(2016QN068). （2016QN068）
