食品工业科技2018，Vol.39Issue(4)：177-182,209,7.DOI:10.13386/j.issn1002-0306.2018.04.033
栉江珧粗多糖提取工艺优化、组成分析及其抗氧化活性研究
Optimization of extraction technology, composition analysis and antioxidant activity of crude polysaccharides from Atrina pectinata
摘要
Abstract
Based on single-factor experiments,response surface methodology (RSM) was employed to optimize the water extraction process of crude Atrina pectinata polysaccharides (CAPPS).The constitute and antioxidant activity of CAPPS obtained by the optimized process were analyzed.The optimal extraction conditions were found to be extraction duration of 215 min at 94 ℃ with a water/material ratio of 34∶1 (mL/g).The yield of crude polysaccharides under the optimized conditions was 18.37％.The contents of polysaccharide,protein,uronic acid and sulfate were 94.39％,2.8％,0.63％ and 0.08％ respectively.Structure and monosaccharide composition of crude polysaccharide were determined by infrared multispectral scanner and chromatographic analysis.And the results showed that crude CAPPS was made of glucose.The results indicated that CAPPS has the eliminating activity on superoxide anion free radicals and hydroxyl radical,as well as metal-chelating ability,and IC50 (the half-inhibitory concentration)values were 0.599 mg/mI,1.01 mg/mL and 0.29 mg/mL respectively.The results in the current study would provide a theoretic basis for future studies on activity and development of functional products with CAPPS.关键词
栉江珧粗多糖/提取工艺/响应面/组成分析/抗氧化活性Key words
crude polysaccharide of Atrina pectinata/extraction technology/response surface methodology/composition analysis/antioxidant activity分类
轻工纺织引用本文复制引用
李松林,王林,叶华,白青云,顾佳秋,顾玉磊,陈晓明..栉江珧粗多糖提取工艺优化、组成分析及其抗氧化活性研究[J].食品工业科技,2018,39(4):177-182,209,7.基金项目
淮阴工学院研究生科技创新计划基金项目(HGYK201502). （HGYK201502）
