食品工业科技2018，Vol.39Issue(4)：210-214,5.DOI:10.13386/j.issn1002-0306.2018.04.038
高效液相色谱法测定红小丑鱼中虾青素和叶黄素含量
Determination of lutein and astaxanthin in Amphiprion frenatus by reversed-phase high performance liquid chromatography
摘要
Abstract
A high performance liquid chromatography method was established for the determination of lutein and astaxanthin in Amphiprionfrenatus.The sample was extracted by acetone and methyl tertiary butyl ether,and cleaned up by an LC-NH2 column.The chromatographic separation was achieved on C18 column used amobile phase made up of methanol and water (98∶2,v/v) by equal gradient elution.The detection wave length was set at 475 nm,and an external standard method was used to quantify astaxanthin and lutein.An excellent linear relationship of this method for astaxanthin and lutein were observed with values of 0.998 and 0.999.The average spike recovery rates of lutein and astaxanthin were 79.92％ ～90.32％ and 78.51％ ～97.60％ with relative standard deviations of 6.35％～7.07％ and 10.79％～13.41％.This method was sensitive,repeatable and suitable for the determination of astaxanthin and lutein in small ornamental fish.关键词
高效液相色谱法/虾青素/叶黄素/红小丑鱼Key words
reversed-phase high performance liquid chromatography (HPLC)/astaxanthin/lutein/Amphiprion frenatus分类
轻工纺织引用本文复制引用
张芬,杜虹,汤水粉,刘海新,林琪,朱志煌..高效液相色谱法测定红小丑鱼中虾青素和叶黄素含量[J].食品工业科技,2018,39(4):210-214,5.基金项目
福建省科技厅省属公益类专项项目(2016R1003-1). （2016R1003-1）
