食品工业科技2018，Vol.39Issue(4)：235-239,5.DOI:10.13386/j.issn1002-0306.2018.04.043
流式细胞技术检测酸性饮料中菌落总数的研究
Study on total plate count detection in acid beverage by flow cytometry
张兰 1徐红 1翁文川 2凌莉 2冼钰茵 2杨铭燕 1潘斯威 1黄焯枝1
作者信息
- 1. 达能(中国)食品饮料有限公司,广东中山528415
- 2. 广东出入境检验检疫技术中心,广东省动植物与食品进出口技术措施研究重点实验室,广东广州510000
- 折叠
摘要
Abstract
Compared and analyzed with pour plate method and membrane filtration method,flow cytometer(FCM) was used to determine total plate count (TPC)for establishing a FCM method in rapid detection of TPC in acid beverage (pH ＜ 4.6).13 strains were selected to conduct artificial add experiment.The results showed that substrate and concentration had great influence on FCM method,some bacteria in acid beverage could not be detected by FCM method directly,which need to be filtrated on membrane and pre-enriched in neutral TSB broth at 36 ℃ for 22 h,then detected by FCM and pour plate method,results were at the same order of magnitude.The official AOAC analysis method was used to do the statistics analysis on qualitative result of 13 strains in acid beverage with different concentration detected by membrane filtration method and FCM method after pre-enrichment for 22 h,X2 ＜ 3.84 and false negative rate was 1.9％ ＜ 2％,false positive rate was 4.2％ ＜ 9.6％,which showed that membrane filtration method could be replaced by FCM method for TPC qualitative detection in acid beverage,then used for products quick release.关键词
酸性饮料/流式细胞技术/菌落总数/平板法/滤膜法Key words
acid beverage/FCM/TPC/pour plate method/membrane filtration method分类
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张兰,徐红,翁文川,凌莉,冼钰茵,杨铭燕,潘斯威,黄焯枝..流式细胞技术检测酸性饮料中菌落总数的研究[J].食品工业科技,2018,39(4):235-239,5.
