食品与机械2017，Vol.33Issue(12)：76-79,4.DOI:10.13652/j.issn.1003-5788.2017.12.015
白及萃取物的抑菌活性及其二氯甲烷萃取物化学成分分析
Antibacterial activity of fractions and chemical constituents analysis of dichloromethane fraction from BIetiIIa striata
摘要
Abstract
Bletilla striata extracts were prepared by methanol cir-cumfluence extraction.Five different polarity fractions were obtained by extraction in turn with n-hexane,dichloromethane,ethyl acetate,n-butanol and water.The inhibiting effects on E.coli,S.aureus,B. subtilis and P.aeruginos were measured by using filter paper disc diffusion method and minimum inhibitory concentration (MIC) method.The chemical constituents of the active fraction were analyzed by gas chromatography-mass spectrometry (GC-MS).The results showed that the methanol extract,n-hexane and dichlo-romethane fractions exhibited significant inhibition against four com-mon bacterial strains.Dichloromethane fraction presented the best antibacterial effect than other fractions (P<0.05).Dichloromethane fraction exerted potent inhibitory effects on E.coli,S.aureus,B. subtilis and P.aeruginos in a dose-dependent manner.Its MIC against four common bacterial strains was 2 mg/mL.Eleven com-pounds representing 94.16% of the total constituents were identified by GC-MS analysis.The main constituents of dichloromethane fraction were 2-methyl-3,4-benzophenanthrene (39.97%),ethyl ho-movanillate (25.25%),trans-4′-fluoro-4-(methylthio) chalcone (9.44%),dioctyl sebacate (7.34%),and linoleic acid (3.86%).In conclusion,B.striata fractions exerted potent antibacterial activity. Dichloromethane fraction was found to be the most active,and aro-matic series,esters,organic acids might be potential bioactive com-ponents.关键词
白及/二氯甲烷萃取物/抑菌/GC-MSKey words
Bletilla striata/dichloromethane fraction/antibacterial/GC-MS引用本文复制引用
吴永祥,程满怀,江海涛,侯书增,万志兵..白及萃取物的抑菌活性及其二氯甲烷萃取物化学成分分析[J].食品与机械,2017,33(12):76-79,4.基金项目
安徽省留学回国人员创新项目择优资助计划重点项目(编号:2017srst1) （编号:2017srst1）
校企合作项目 (编号:hxkt20170015, hxkt20170016) （编号:hxkt20170015, hxkt20170016）
安徽省高校自然科学研究重点项目(编号:KJ2017A398) （编号:KJ2017A398）
