食品与机械2017，Vol.33Issue(12)：129-134,6.DOI:10.13652/j.issn.1003-5788.2017.12.026
绿茶水粗提物抗中波紫外线致人表皮细胞衰老作用
Effect of anti-aging on human epidermal keratinocyte by UVB induced for green tea crude water extract
摘要
Abstract
In this study,anti-aging effects of green tea crude water extract were studied using human epidermal keratinocyte as model which induced by ultraviolet B (UVB).The green tea extract of 0~20 μg/mL was added to DMEM cell culture medium,then cells were induced by radiation of 60 mJ/cm2UVB.The anti-aging effects were studied by cellular morphology,apoptosis level,cell viability,intra-cellular antioxidant enzyme activity,intracellular active oxygen cluster (ROS)content,malonaldehyde(MDA)content,cell mito-chondrial membrane potential and Na+,K+-ATP enzyme activities. The results showed that the crude extract of green tea could obviously improve cell volume,cell shape,and nuclear volume of in-duced cells.Apoptosis of cells was inhibited,and the survival rate was increased by (30.52 ± 8.69)%.The activity of antioxidant enzyme in cells was obviously improved.ROS content was decreased by 17.16%.The content of MDA was reduced by (50.69±6.81)%. The average light density of red green fluorescence in JC-1 dyeing was increased by 13.75%.Besides,the activity of Na+,K+-ATP en-zyme was increased by (103.95±3.64)%.The experimental results show that green tea extracts have anti-UVB induced aging effect on human epidermal cell,and this study may provide scientific basis to develop anti-aging products of green tea.关键词
绿茶/水粗提物/中波紫外线/人表皮角质形成细胞/抗衰老Key words
green tea/water crude extract/ultraviolet radiation B/HaCaT/anti-aging引用本文复制引用
王振,刘仲华,蔡淑贤,刘安,文祎..绿茶水粗提物抗中波紫外线致人表皮细胞衰老作用[J].食品与机械,2017,33(12):129-134,6.基金项目
国家自然科学基金项目(编号:31471590) （编号:31471590）
十三五规划项目(编号:CARS-23-03A,CARS-23-09B,ARS-23-C) （编号:CARS-23-03A,CARS-23-09B,ARS-23-C）
