食品与机械2017，Vol.33Issue(12)：154-159,6.DOI:10.13652/j.issn.1003-5788.2017.12.031
齐口裂腹鱼骨胶原蛋白超声波辅助提取工艺及其特性研究
Ultrasonic-assisted extraction process and characteristics analysis of col lagen from Schizothorax prenanti bone
摘要
Abstract
The ultrasonic-assisted acid extraction technology of colla-gen from Schizothorax prenanti bone was studied using univariate a-nalysis and orthogonal test to improve the extraction rate.The puri-fied collagen was analyzed by the methods of polyacrylamide gel elec-trophoresis,ultraviolet spectroscopy,amino acid and denaturation temperature analysis.The results showed that ultrasonic pretreat-ment increased the collagen extraction rate significantly.The optimal extracting conditions were as followed: extracting temperature 30 ℃,ratio of,liquid-solid 75︰1 (mL/g),time for pre-treating by the ultrasonic 20 min,and extracted time.In the conditions,the col-lagen extraction ratio was 6.91% and the purity reached 89.74%.In addition,SDS-PAGE results suggested that the collagen was com-prised ofα1,α2andβchain,which might be classified as type I colla-gen.Maximum UV absorption wavelength of collagen was between 220~232 nm.Schizothorax prenanti bone collagen contained high percentages of Gly (3 1.2 1 % of total amino acid), and its denaturation temperature was 3 1.4 ℃.关键词
鱼骨/胶原蛋白/超声波/酸法/特性Key words
fishbone/collagen/ultrasonic/acid method/characteristics引用本文复制引用
严秋萍,邬应龙,李月娥,蒋艳,郭玉春,秦涛..齐口裂腹鱼骨胶原蛋白超声波辅助提取工艺及其特性研究[J].食品与机械,2017,33(12):154-159,6.基金项目
四川农业大学"211"工程双支计划(2016)(编号:03571003) （2016）
