食品与机械2017，Vol.33Issue(12)：191-196,6.DOI:10.13652/j.issn.1003-5788.2017.12.038
食源性致病菌PCR检测前处理方法研究进展
A review of the development in pretreatment methods for PCR detection of food-borne pathogens
摘要
Abstract
Molecular detection methods based on PCR (polymerase chain reaction)are widely used in the rapid detection of food-borne pathogens.However,in practical applications pretreatment method is also a critical step in rapid detection except the optimization of PCR.In this review,the pretreatment methods (including the cen-trifugation,membrane filtration,magnetic separation)for PCR de-tection of food-borne pathogens were systematically summeried.In particular,the magnetic separation was emphatically reviewed in the PCR detection of food-borne pathogens.关键词
食源性致病菌/前处理/聚合酶链式反应/离心/膜分离/磁性分离Key words
foodborne pathogens/pretreatment/PCR/centrifuga-tion/membrane separation/magnetic separation引用本文复制引用
白亚龙,索玉娟,周昌艳..食源性致病菌PCR检测前处理方法研究进展[J].食品与机械,2017,33(12):191-196,6.基金项目
国家农产品质量安全风险评估项目计划 (编号:GJFP201701304) （编号:GJFP201701304）
上海市科技兴农重点攻关项目[编号:沪农科攻字(2015)第4-5号] （2015）
上海市农委(兴农推广-成长计划)[编号:沪农青字(2017)第1-28号] （兴农推广-成长计划）
