中国食品学报2018，Vol.18Issue(2)：1-14,14.DOI:10.16429/j.1009-7848.2018.02.001
谷物食品总黄酮比色法定量的问题及选用原则
The Problem and Selection of the Colorimetric Quantification of Flavonoids of Cereal Foods
摘要
Abstract
More and more studies have shown that cereals were rich in a variety of phenolic compounds and the flavonoids with plenty of biological activities occupied an important part.Thus,accurate quantification of total flavonoid contents was very important for the judgement of food nutritional value and the development of functional food.Spectmphotometric analysis was the most commonly adopted method for quantification of flavonoids,among which NaNO2-Al3+-NaOH method,aluminum chloride method and 2,4-dinitrophenylhydrazine method were frequently used.But flavonoids varied in different cereal food,which increased the difficulty of method selecting and accurate quantification.Therefore,this paper summarizes the statistics of flavonoid composition and contents distribution among different types of grains.According to the absorption of flavonoids under different determination methods,it points out the possible misuse and meanwhile suggests the most appropriate proposal,which will be helpful to enhance the accuracy of flavonoids determination and as a reference in fruit and vegetable and functional food industry.关键词
谷物/比色法/总黄酮含量/NaNO2-Al3+-NaOH法/AlCl3法/2,4-二硝基苯肼法Key words
cereals/colorimetric method/total flavonoid contents/NaNO2-Al3+-NaOH method/aluminum chloride method/2,4-dinitrophenylhydrazine method引用本文复制引用
叶兴乾,沈淑妤,黄睿,吴文艳,周声怡,陈士国..谷物食品总黄酮比色法定量的问题及选用原则[J].中国食品学报,2018,18(2):1-14,14.基金项目
国家自然科学基金项目(31471592) （31471592）
新疆自治区科技项目(2016A03008) （2016A03008）
