茶叶科学2018，Vol.38Issue(2)：174-182,9.
紫娟茶树叶片不同发育期花青素积累及合成相关基因的表达
Anthocyanin Accumulation and Expression of Synthesis-related Genes in Leaves of Different Developmental Stages in Camellia sinensis cv. Zijuan
摘要
Abstract
In order to explore the regulatory factors and structural genes of anthocyanin biosynthesis in leaves of different developmental stages in C. sinensis cv. Zijuan, anthocyanin contents and compositions were analyzed by high performance liquid chromatography-mass, and the expression of synthesis-related genes were tested by RNA-sequencing and digital gene expression (DGE) profiling technology. HPLC-MS analysis showed that the anthocyanin content was consistent with color changes, increased firstly and then decreased with the leaf positions of Zijuan, with the highest anthocyanin content in the second leaf (9.87 mg·g-1) and the lowest content in mature leaf (0.11 mg·g-1). DGE determination results showed that the expression levels of PAL gene were relatively high in bud, second leaf and open surface leaf,but low in mature leaf.C4H,4CL,CHS,CHI,F3H,F3'H,F3'5'H and ANS showed a pattern of declining expression as PAL in different leaf positions of Zijuan. FLS was firstly up-regulated in the second leaf and then declined in the mature leaf, which was consistent to the anthocyanin changes. DFR showed no clear expression pattern. GT and ACT exhibited similar expression patterns, which were up-regulated in the second, open surface and mature leaves.ANR and LAR showed similar expression patterns,which were high in bud,second and open surface leaves, but low in mature leaf. The gene expression of bHLH, MYB and WDR showed different expression patterns in different leaf positons of Zijuan. These results suggested that the temporal expression specificities of structural and regulatory genes may significantly affect the accumulation of anthocyanin in Zijuan.关键词
茶树品种/花青素/数字表达普/差异表达基因Key words
tea cultivar/anthocyanin/digital expression patterns/differentially expressed genes分类
农业科技引用本文复制引用
蒋会兵,孙云南,李梅,戴伟东,宋维希,田易萍,夏丽飞,陈林波..紫娟茶树叶片不同发育期花青素积累及合成相关基因的表达[J].茶叶科学,2018,38(2):174-182,9.基金项目
国家自然科学基金(31560220,31460216)、云南省人才培养计划(2015HB105) （31560220,31460216）
