茶叶科学2018，Vol.38Issue(2)：183-192,10.
茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应
Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress
摘要
Abstract
Sucrose non-fermenting-1-related protein kinase (SnRK) is a kind of serine/threonine protein kinases widely exist in plants. Among them, members of SnRK2 family play a vital role in plant response to stresses. In order to investigate the molecular mechanisms of Camellia sinensis SnRK2 in response to abiotic stresses, two SnRK2 genes from tea plant were cloned and named as CsSnRK2.1 (GenBank accession code: MG026837) and CsSnRK2.2 (GenBank accession code: MF662805) respectively. Bioinformatics analysis showed that they contained 358 and 337 amino acids respectively, which harbored a conserved ATP binding site and Ser/Thr kinase domain and were highly similar to SnRK2 protein kinase of Arabidopsis thaliana and maize. The expression of CsSnRK2.1 was transiently induced and then decreased by high salinity (100 mmol·L-1NaCl), drought (20% PEG6000) and ABA(100 μmol·L-1) stress, but showed no significant changes under low (4℃) or high temperature treatments (38℃). By contrast, CsSnRK2.2 was strongly induced by high salt and temperature treatments. The results revealed that CsSnRK2.1 and CsSnRK2.2 might be closely related to stress responses in tea plant.关键词
茶树/SnRK2s/克隆/非生物胁迫Key words
tea plant(Camellia sinensis)/SnRK2s/cloning/abiotic stress分类
农业科技引用本文复制引用
张永恒,万思卿,陈江飞,王伟东,周天山,肖斌,杨亚军,余有本..茶树CsSnRK2.1、CsSnRK2.2基因的克隆及在非生物胁迫中的响应[J].茶叶科学,2018,38(2):183-192,10.基金项目
国家茶叶产业技术体系、汉中综合试验站(CARS-23)、陕西省农业专项资金项目(tg2015-099)、中国博士后科学基金面上项目(2016M602873) （CARS-23）
