肉类研究2018，Vol.32Issue(2)：33-39,7.DOI:10.7506/rlyj1001-8123-201802006
基于SELDI-TOF-MS的冷却滩羊肉微生物差异蛋白分析
Surface Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry Analysis of Surface Microbial Differential Proteins in Chilled Tan Sheep Meat
摘要
Abstract
Microbial proteins on the surface of tray-packaged chilled Tan sheep meat were analyzed by surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and biological samples collected at different times during chilled storage were differentiated. The variable importance in the projection (VIP) value (threshold > 1) of the first principal component of the orthogonal partial least squares-discriminant analysis (OPLS-DA) model was determined and combined with the P value (threshold < 0.05) of student's t test to find out the differentially expressed proteins. The results showed that 132 differential protein peaks were obtained by Biomarker Wizard software, 75 of which were identified by searching in the Swiss-Prot database based on their mass-to-charge ratio, and the remaining 57 were unknown proteins. These differential proteins did not include enzymes but included 19 proteins that might associated with bacteria on mutton meat. In summary, SELDI-TOF-MS allows the detection of various differentially expressed microbial proteins at different storage times of chilled Tan sheep meat and consequently the identification of a large number of valuable protein markers.关键词
滩羊肉/微生物/表面增强激光解吸电离飞行时间质谱/差异蛋白Key words
chilled Tan sheep meat/microorganism/surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS)/differential protein分类
轻工纺织引用本文复制引用
胡倩倩,杨波,罗瑞明,尤丽琴,李子欣..基于SELDI-TOF-MS的冷却滩羊肉微生物差异蛋白分析[J].肉类研究,2018,32(2):33-39,7.基金项目
国家自然科学基金地区科学基金项目(31460431) （31460431）
