食品与机械2017，Vol.33Issue(10)：6-10,5.DOI:10.13652/j.issn.1003-5788.2017.10.001
泡菜中乳酸菌的分离鉴定及体外抗性筛选
Isolation and identification of lactic acid bacteria from fermented vegetables and in vitro resistance screening
摘要
Abstract
This study aimed to isolate lactic acid bacteria containing in vitro resistance ability from Sichuan fermented vegetables.Five strains of Lactobacillus were isolated from fermented vegetables and analyzed by 16S rDNA.Four strains of them were Lactobacillus plantarum,named as E-6-1,E-1-2,E-6-3,D-2-3 respectively,and another one was Lactobacillus alimentarius,named as C-2-1.The in vitro resistance of the 5 strains was determined by artificial gastric juice and bile salt.The results showed that the lactic acid bacteria numbered D-2-3 had good anti-artificial gastric juice ability,and the survival rate was 95.38％ after 3 h of artificial gastric juice treatment at pH 3.0,growing well at 1,2 and 3 g/L bile salt.These showed that the strain has probiotic potential,through further verification was expected to carry out further development and utilization.关键词
泡菜/乳酸菌/分离鉴定/抗性筛选/16S rDNAKey words
fermented vegetables/lactic acid bacteria/isolation and identification/resistance screening/16S rDNA引用本文复制引用
周先容,兰凌霞,汤艳燕,黄盛蓝,李玉珠,于丽洪,Zsolt Zalán,赵欣,杜木英..泡菜中乳酸菌的分离鉴定及体外抗性筛选[J].食品与机械,2017,33(10):6-10,5.基金项目
中央高校基本科研业务费专项资金(编号:XDJK2017D133) （编号:XDJK2017D133）
中央高校基本科研业务费专项资金(编号:XDJK2017D125) （编号:XDJK2017D125）
