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基于454方法分析曲霉型豆豉发酵中细菌群落多样性

李世瑞 石聪 蒋立文 廖卢艳 周红丽 李跑

食品与机械2017,Vol.33Issue(10):11-15,118,6.
食品与机械2017,Vol.33Issue(10):11-15,118,6.DOI:10.13652/j.issn.1003-5788.2017.10.001

基于454方法分析曲霉型豆豉发酵中细菌群落多样性

Analyzing of bacterial diversity involved in Aspergillus-type Douchi by 454 pyrosequencing

李世瑞 1石聪 2蒋立文 1廖卢艳 2周红丽 1李跑1

作者信息

  • 1. 食品科学与生物技术湖南省重点实验室,湖南长沙410128
  • 2. 湖南农业大学食品科技学院,湖南长沙410128
  • 折叠

摘要

Abstract

The changes of bacterial diversity in eight stages of Asper-gillus fermented bean curd fermentation were determined by 454 high-throughput pyrosequencing technique.The changes and diversity of bacterial community at different stages were studied.The results showed that the similarity of the major microorganisms in different samples at the two levels was high,with a total of more than 90% of the total,including three types of Phytophagous,Proteobacteria and Actinomycetes.The samples with the highest level of homogeneity were HY04,and 31 major genera were found in it.It was also found that the abundance of Weissella,Bacillus,Staphylococcus,Lactococcus,Enterococcus and Pseudomonas were different in different fermentation stages appears.The results showed that the microbial community structure was complicated during the fermentation of Aspergillus flavus,and the main flora was relatively stable.However,the relationship among flora,quality and flavor,safety evaluation deserved further attention.

关键词

豆豉/曲霉型/微生物群落/多样性/高通量测序

Key words

Douchi/aspergillus/microbial community/diversity/high-throughput sequencing

引用本文复制引用

李世瑞,石聪,蒋立文,廖卢艳,周红丽,李跑..基于454方法分析曲霉型豆豉发酵中细菌群落多样性[J].食品与机械,2017,33(10):11-15,118,6.

基金项目

国家自然科学基金项目(编号:31371828) (编号:31371828)

食品与机械

OA北大核心CSCDCSTPCD

1003-5788

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