食品与机械2017，Vol.33Issue(10)：34-38,51,6.DOI:10.13652/j.issn.1003-5788.2017.10.001
唾液链球菌抑制变形链球菌生物膜作用的发酵条件优化
Optimal cultivation conditions for Streptococcus salivarius BD3900 to express substances capable of inhibiting Streptococcus mutans biofilm formation
摘要
Abstract
In order to increase the metabolites of the strain BD3900 inhibiting the S.mutans biofilm formation,the inhibition rate of biofilm formation by Streptococcus mutans was used as indexes.The effects of the cultivation time and temperature,the carbon resources,the concentration of sucrose and the inoculum amount were investigated.The results showed that optimal cultivation conditions were composed of a cultivation period of 16 h,a cultivation temperature of 34 ℃,and a sucrose concentration of 1 g/100 mL.Under the optimized cultivation conditions,the inhibitory ability of the strain BD3900 on the formation of S.mutans biofilm was increased by 10％,compared with the results before optimization.The mechanism involved in the inhibiting of S.mutans biofilm formation by the strain BD3900 was probably decreasing the insoluble exopolysaccharides synthetization.关键词
唾液链球菌BD3900/变形链球菌/生物膜形成量/培养条件/不溶性胞外多糖Key words
Streptococcus salivarius BD3900/Streptococcus mutans/biofilm formation/cultivation conditions/insoluble exopolysaccharide引用本文复制引用
于亚男,吴正钧,韩瑨..唾液链球菌抑制变形链球菌生物膜作用的发酵条件优化[J].食品与机械,2017,33(10):34-38,51,6.基金项目
上海市闵行区重大产业技术攻关项目(编号:2015MH093) （编号:2015MH093）
上海乳业生物工程技术研究中心(编号:16DZ2280600) （编号:16DZ2280600）
