食品与机械2017，Vol.33Issue(10)：123-127,144,6.DOI:10.13652/j.issn.1003-5788.2017.10.001
重组抗冻肽在乳酸乳球菌中表达及其抗冻活性研究
Expression of recombinant antifreeze peptide in Lactococcus lactis and its antifreeze activity
摘要
Abstract
In order to optimize the expression of antifreeze protein SFP,using Nisin as the inducer to induced the expression of recombinant Lactococcus lactis.The expression conditions of induction time,induced pH,induced temperature and inducer Nisin concentration were optimized.The optimal expression conditions were determined by SDS-PAGE and Western blot.Furthermore,the physiological characteristics of recombinant bacteria under freezing stress were investigated by comparing the growth status,the acid production of bacteria and the changes of sodium and potassium contents before and after freezing stress.The results showed that the optimal expression conditions were determined:pH of 7.0,Nisin concentration of 15 ng/mL,temperature of 25 ℃,and incubation time of 6 h.The recombinant strain SF-P2 could significantly improved the growth of Lactococcus lactis in the logarithmic phase and the stable growth lag following freezing,and had a significant improvement in fermentation of L.lactis following freezing.It also could effectively reduced the effect of freezing stress on the permeability of cell membrane,which played a role in protecting the physiological function of cells.The results showed that the recombinant strain SFP2 had significant protective effects against cold stress.关键词
抗冻肽/乳酸乳球菌/重组表达/抗冻活性/冷冻胁迫/生理功能Key words
Antifreeze peptide/Lactococcus lactis/Recombinant expression/Antifreeze activity/Freezing stress/Physiological function引用本文复制引用
张莉,黄心阳,马添熠,吴金鸿,汪少芸,王正武..重组抗冻肽在乳酸乳球菌中表达及其抗冻活性研究[J].食品与机械,2017,33(10):123-127,144,6.基金项目
国家自然科学基金项目(编号:31471623,21276154) （编号:31471623,21276154）
国家重点研发计划项目(编号:2016YFD0400206) （编号:2016YFD0400206）
