食品与机械2017，Vol.33Issue(10)：145-148,162,5.DOI:10.13652/j.issn.1003-5788.2017.10.001
苦瓜渣蛋白提取工艺优化及其体外抑制K562细胞增殖作用研究
Optimization of extraction technology of protein from Momordica charantia residue and its inhibitory effect on proliferation of K562 cells in vitro
摘要
Abstract
Single factor combined with uniform design were used to optimized the extraction process of protein from Momordica charantia residue by salt dissolving method,and the inhibitory activity in vitroon K562 cell proliferation of crude extract was determined by MTT assay.The test results showed that the optimal conditions were as followed:salt concentration of 0.25 mol/L,time 3.59 h,pH=9,ratio of liquid-material 10.36 ∶ 1 (mL/g) and the temperature of 25 ℃,extracting twice.Under this condition,the actual extraction rate was 9.28％.In the range of experimental concentration,the crude protein from Momordica charantia residue had a significant inhibitory effect on proliferation of K562 cells in vitro,and showed dose-effect relationship.关键词
苦瓜渣/蛋白/提取工艺/K562细胞/增殖抑制Key words
Momordica charantia residue/protein/extraction process/K562 cell/proliferation inhibition引用本文复制引用
李镁娟,郝景灏,马甜甜,时国庆,张新武,黄继红..苦瓜渣蛋白提取工艺优化及其体外抑制K562细胞增殖作用研究[J].食品与机械,2017,33(10):145-148,162,5.基金项目
郑州市国际科技合作与交流项目(编号:141PGJHZ546) （编号:141PGJHZ546）
