食品与机械2017，Vol.33Issue(10)：149-153,5.DOI:10.13652/j.issn.1003-5788.2017.10.001
提取方法对北沙参多糖性质及生物活性的影响
Effect of different extraction methods on the properties and biological activity of polysaccharides from Radix Glehniae
摘要
Abstract
The polysaccharides were extracted from Radix Glehniae,by using hot water extraction,ultrasonic extraction,microwave extraction,lye extraction,acid extraction and cellulase extraction,and the corresponding polysaccharide was GLP-W,GLP-U,GLP-M,GLP-L,GLP-A and GLP-E.The extraction rate,soluble sugar content,viscosity,structural characteristics,solid morphology,antioxidant activity and hypoglycemic activity of each polysaccharide were measured.The results showed:the extraction rates of polysaccharides were GLP-A(39.62 ％) ＞GLP-E(38.33 ％) ＞ GLP-L(24.80 ％) ＞ GLP-W(23.93％) ＞GLP-M(18.92％)＞GLP-U(12.68％);The viscosity of the polysaccharide solution by different extraction methods were increased with the increase of polysaccharide solution concentration,and at the same concentration,GLP-L had the largest viscosity.The content of soluble sugar was GLP-A (74.05％)＞ GLP-L (67.57％) ＞ GLP-W (65.41％) ＞ GLP-U (63.25％) ＞ GLP-E (33.03 ％) ＞ GLP-M (28.71％).The antioxidant activity and the inhibitory activity against α-glucosidase showed that,with the increase of polysaccha-ride concentration,the activity gradually increased.However,when the concentration reached a certain range,the activity tended to be stable.Ultrasonic extraction of R.Glehniae polysaccharide scavenging of DPPH and hydroxyl radicals was the highest,and the IC50 were 3.091 mg/mL and 1.999 mg/mL,respectively;The strongest inhibition of α-glucosidase was obtained by alkali extraction,the IC50 was 1.437 mg/mL.关键词
北沙参/多糖/表征/抗氧化/α-葡萄糖苷酶抑制剂Key words
Radix Glehniae/polysaccharide/characterization/antioxidation/a-glucosidase inhibitor引用本文复制引用
景永帅,张丹参,张瑞娟,苏蕾,潘孟树,孙帅帅,吴兰芳,郑玉光..提取方法对北沙参多糖性质及生物活性的影响[J].食品与机械,2017,33(10):149-153,5.基金项目
河北省自然科学基金项目(编号:H2016208059,H2016208058,H2017423023) （编号:H2016208059,H2016208058,H2017423023）
河北省高等学校科学技术研究项目(编号:ZD2016009,QN2016187) （编号:ZD2016009,QN2016187）
河北省现代农业技术体系中药材产业创新团队建设(编号:20170401458-2) （编号:20170401458-2）
