食品科学技术学报2018，Vol.36Issue(2)：52-57,6.DOI:10.3969/j.issn.2095-6002.2018.02.007
香菇柄中5'-核苷酸的酶法提取及组成分析
Enzymatic Extraction and Composition Analysis of 5'-Nucleotides from Shiitake Mushroom Stipe
摘要
Abstract
To improve the yield of 5'-nucleotides and increase the economic value of shiitake mushroom stipe,5'-nucleotides were extracted from shiitake mushroom stipe by cellulase. The effects of cellulase amount,liquid-solid ratio, enzymatic hydrolysis temperature, enzymatic hydrolysis time and pH on the yield of 5'-nucleotides were investigated by single factor experiments,and the orthogonal test was used to optimize the parameters. The 5'-nucleotides composition was also analyzed by high performance liquid chromatography (HPLC). Results showed that on the basis of single factor experiments, the parameters of cellulase extraction on 5'-nucleotides optimized by orthogonal test were as follows: liquid-solid ratio 20:1 (mL:g), cellulase amount 0.8%, enzymatic hydrolysis temperature 40 ℃, enzymatic hydrolysis time 4 h,and pH 5.4. Under these optimum conditions, the yield of 5'-nucleotides was 4.08 mg·g-1. This result was close to the content (4.57 mg·g-1) measured by HPLC. The 5'-nucleotides extracted from shiitake mushroom stipe using cellulase were composed by 5'-guanosine monophosphate,5'-uridine monophosphate, 5'-cytosine monophosphate, 5'-adenosine monophosphate, 5'-inosine monophosphate, 5'-xanthosine monophosphate,and umami taste nucleotides accounted for 62.63% of 5'-nucleotides.关键词
香菇柄/纤维素酶/提取/5'-核苷酸/高效液相色谱/组成分析Key words
shiitake mushroom stipe/cellulase/extraction/5'-nucleotides/HPLC/components analysis分类
轻工纺织引用本文复制引用
李顺峰,王安建,张雪彦,田广瑞,刘丽娜,魏书信,高帅平..香菇柄中5'-核苷酸的酶法提取及组成分析[J].食品科学技术学报,2018,36(2):52-57,6.基金项目
河南省财政预算项目(豫财预[2017]76-18). （豫财预[2017]76-18）
