食品科学2018，Vol.39Issue(7)：78-84,7.DOI:10.7506/spkx1002-6630-201807012
不同小白菜品种硝酸盐含量、氮代谢关键酶活性及NRT1表达和亚细胞定位
Nitrate Content, Activities of Key Enzymes for Nitrogen Metabolism, and Expression and Subcellular Localization of NRT1 in Different Nitrate-Enriched Varieties of Pakchoi(Brassica chinensis L.)
摘要
Abstract
Solution culture experiments were conducted to investigate the effect of nitrate/ammonium ratio (50:50, 75:25 and 100:0) on nitrate content, the activities of key enzymes for nitrogen metabolism in pakchoi, and the expression and subcellular localization of nitrate transporter gene in two pakchoi varieties (low-nitrate variety 'JN4' and high-nitrate variety'HKTX'). The results showed that nitrate contents in leaves of 'HKTX' and JN4 subjected to treatment at NO3- /NH4+ ratio of 100:0 increased respectively by 13.2% and 30.4% compared with treatment at NO3-/NH4+ ratio of 150:50; nitrate contents in petioles of 'HKTX' and 'JN4' were increased respectively by 14.3% and 4.9% compared with treatment at NO3- /NH4+ ratio of 50:50. With the increase of NO3- /NH4+ ratio, the activities of nitrate reductase (NR) and glutamate synthase (GOGAT) in pakchoi decreased, the activity of nitrite reductase (NiR) increased, and the activity of glutamate dehydrogenase (GDH) initially increased and then decreased. Significant differences in NR, GOGAT, NiR, and GDH were observed between the two varieties.Significant expression of low affinity transporter gene(NRT1)was detected in both varieties,and a higher expression level was observed in 'JN4' than in 'HKTX'. NRT1 was a low affinity nitrate transporter located in the plant cell membrane.关键词
硝铵比/硝酸盐含量/氮代谢关键酶/硝酸盐转运蛋白基因/亚细胞定位/小白菜Key words
nitrate/ammonium ratio/nitrate content/key enzymes involved in nitrogen metabolism/nitrate transporter gene/subcellular localization/pakchoi分类
农业科技引用本文复制引用
李彦华,秦余丽,王正银,谢德体,杨芸,徐卫红,周鑫斌,王卫中,迟荪琳,李桃,张春来,赵婉伊..不同小白菜品种硝酸盐含量、氮代谢关键酶活性及NRT1表达和亚细胞定位[J].食品科学,2018,39(7):78-84,7.基金项目
国家现代农业(大宗蔬菜)产业技术体系建设专项(CARS-23) （大宗蔬菜）
"十一五"国家科技支撑计划项目(2007BAD87B10) （2007BAD87B10）
