食品科学2018，Vol.39Issue(7)：91-97,7.DOI:10.7506/spkx1002-6630-201807014
大豆糖蛋白的抗氧化特性
Antioxidant Property of Soy Glycoprotein
摘要
Abstract
Soy glycoprotein was prepared from soy protein isolate (SPI) and glucose (m/m = 1:1) by Maillard reaction at 70, 80 or 90 ℃ for 0, 1, 2, 3, 4, 5, 6 h respectively. The reducing power, and hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of products at different reaction times were determined. Moreover, browning degree, ultraviolet (UV) spectroscopic analysis, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were studied to explore the antioxidant mechanism of soy glycoprotein. The results showed that the antioxidant effect and browning degree of soy glycoprotein was significantly increased, and free amino group content was remarkably increased with an increase in reaction temperature and time. It was proved that soy glycoprotein obtained after reaction for 6 h at 90 ℃had the maximum reducing power, which was 5.6 times higher than SPI. Soy glycoprotein obtained after reaction at 90 ℃ for 5 h exhibited the highest hydroxyl radical scavenging (a 1.69-fold increase over SPI) and DPPH radical scavenging activity (scavenging activity of 68.55%, a 3.68-fold enhancement over SPI). Through analysis of the secondary derivative ultraviolet spectrum, tryptophan migration in the protein occurred during the Maillard reaction. Meanwhile, SDS-PAGE illustrated that SPI and glucose formed high molecular weight conjugates. All in all, these results suggest that soy glycoprotein has antioxidant properties, which has a close relationship with the changes of protein structure.关键词
大豆蛋白/美拉德反应/糖蛋白/抗氧化/结构/机理Key words
soybean protein/Maillard reaction/glycoprotein/antioxidant/structure/mechanism分类
轻工纺织引用本文复制引用
夏秀芳,王博,郑幸子,邓思杨,潘男,王浩..大豆糖蛋白的抗氧化特性[J].食品科学,2018,39(7):91-97,7.基金项目
黑龙江应用技术研究与开发计划重大项目(GA15B302) （GA15B302）
黑龙江省博士后资助项目(LBH-15013) （LBH-15013）
