食品科学2018，Vol.39Issue(7)：195-200,6.DOI:10.7506/spkx1002-6630-201807029
牛乳α-乳白蛋白免疫球蛋白G线性表位的关键氨基酸识别
Characterization of Critical Amino Acids in Linear Immunoglobulin G Epitopes of α-Lactalbumin in Cow Milk
摘要
Abstract
α-Lactalbumin is one of the major allergens in cow milk. Characterizing the critical amino acids in the epitopes of α-lactalbumin is of great significance for the understanding of the role of immunoglobulin G (IgG) in milk allergy and the development of hypoallergenic dairy products. In this study, the alanine scanning of immunodominant epitopes was used to identify the critical amino acids. First, a series of peptides based on the sequence of α-lactalbumin were identified with IgG in individual sera from cow milk-allergic patients by enzyme-linked immunosorbent assay (ELISA). Then, new peptides were synthesized by sequential replacement of the amino acids acting on the epitopes with alanine, and the critical amino acids were identified with serum pool from cow milk-allergic patients by ELISA. The results showed that IgG-binding epitopes were located in the sequences of aa 6-20, aa 21-35, aa 36-50 and aa 86-100. Our data revealed Phe9, Leu15, Pro24, Trp26and His32were the critical aa in the IgG-binding epitopes.关键词
牛乳过敏/α-乳白蛋白/免疫球蛋白G作用表位/关键氨基酸Key words
cow milk allergy/α-lactalbumin/immunoglobulin G binding epitope/critical amino acids分类
轻工纺织引用本文复制引用
丛艳君,李晔,刘家琦,陈澍,于晓凤,李林峰..牛乳α-乳白蛋白免疫球蛋白G线性表位的关键氨基酸识别[J].食品科学,2018,39(7):195-200,6.基金项目
国家自然科学基金青年科学基金项目(31101236) （31101236）
北京市自然科学基金项目(6132004) （6132004）
北京市科技新星计划项目(Z131102000413005) （Z131102000413005）
