食品科学2018，Vol.39Issue(7)：215-220,6.DOI:10.7506/spkx1002-6630-201807032
货架期冷藏过程中鳝鱼肉理化指标及蛋白质变化规律
Changes in Physicochemical Properties and Proteins during Shelf Life of Cold Stored Swamp Eel(Monopterus albus)
摘要
Abstract
The purpose of this study was to investigate the quality changes of cold stored Monopterus albus during shelf life. The results showed that the shelf life of Monopterus albus was 4 days during cold storage as determined by measuring total volatile basic nitrogen (TVB-N) content, K value (freshness index) and thiobarbituric acid reactive substances (TBARS). pH decreased firstly and then increased with storage time, color turned from bright red to brown red, and water-holding capacity decreased continuously. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and trichloroacetic acid (TCA)-soluble nitrogen measurement showed that myosin and actin were degraded gradually during storage. Chemical analysis indicated that ionic bonds and hydrogen bonds decreased, whereas disulfide bonds and hydrophobic interactions showed a gradually increasing trend. Fourier transform infrared spectroscopy analysis indicated that amide A band moved to high wavenumber, which was consistent with the changes of hydrogen bonds. Curve fitting of amide I band indicated that interconversion between β-sheet and β-turn was dominant during the early storage period, and then the protein structural components were transformed to α-helix and random coil, indicating increased structural disorderliness. Overall, these results showed that hydrogen bonds and ionic bonds may be the major forces to maintain the secondary structure of protein during the early storage period. The destruction of hydrogen bonds and ionic bonds can cause changes in protein conformation.关键词
鳝鱼/冷藏/货架期/理化性质/蛋白质Key words
Monopterus albus/cold storage/shelf life/physicochemical properties/protein分类
农业科技引用本文复制引用
郑红,苏现波,马良,张晓洁,马明思,孙艺,张宇昊..货架期冷藏过程中鳝鱼肉理化指标及蛋白质变化规律[J].食品科学,2018,39(7):215-220,6.基金项目
"十三五"国家重点研发计划重点专项(2016YFD0400200) （2016YFD0400200）
国家自然科学基金面上项目(31671881) （31671881）
中国博士后科学基金特别资助项目(2015T80951) （2015T80951）
重庆市基础科学与前沿技术研究重点项目(cstc2015jcyjBX0116) （cstc2015jcyjBX0116）
第四批重庆市高等学校优秀人才支持计划项目 （）
