食品科学2018，Vol.39Issue(7)：329-337,9.DOI:10.7506/spkx1002-6630-201807048
微生物脂肪酶资源挖掘研究进展
Progress in Mining of Microbial Lipase Resources
摘要
Abstract
Lipases are ubiquitous in animals, plants and microorganisms, but industrial lipases are mainly produced by microorganisms. The industrial application of lipases is constrained by its high cost and enzymatic characteristics such as temperature and pH tolerance, specific activity, substrate specificity and solvent resistance. Despite the abundance of information regarding microbial lipase genes and proteins, there are currently very few commercial lipase preparations that are suitable for application in the food, pharmaceutical and energy industries. Accordingly, tremendous research efforts have been made to mine novel alternative lipase resources. This article mainly presents a review of the approaches used to mine microbial lipase resources including environmental screening and metagenomics screening for mining new lipases with good properties, genetic improvement of lipase-producing strains, recombinant expression of lipases in genetically engineered strains, lipase modification by protein engineering, lipase immobilization and optimization of reaction conditions of immobilized lipase.关键词
微生物脂肪酶/资源挖掘/环境筛选/重组表达/蛋白质工程Key words
microbial lipase/resource mining/environmental screening/recombinant expression/protein engineering分类
生物科学引用本文复制引用
蔡海莺,王珍珍,张婷,赵敏洁,李杨,毛建卫,冯凤琴..微生物脂肪酶资源挖掘研究进展[J].食品科学,2018,39(7):329-337,9.基金项目
浙江省重大科技专项(2012C12005-2) （2012C12005-2）
浙江省自然科学基金一般项目(LY18C200006) （LY18C200006）
浙江省教育厅科研一般项目(Y201737161) （Y201737161）
浙江省农业生物资源生化制造协同创新中心开放基金项目(2016KF0048) （2016KF0048）
