食品科学2018，Vol.39Issue(8)：45-49,5.DOI:10.7506/spkx1002-6630-201808008
mok E基因过表达对红曲霉Monacolin K产量、菌丝及孢子形态的影响
Effect of mok E Overexpression on Monacolin K Production and Morphology of Mycelia and Spores in Monascus
摘要
Abstract
mok E gene belongs to the Monacolin K biosynthesis gene cluster,whose expression level is positively correlated with Monacolin K production.In this research,mok E gene was overexpressed in Monascus M1 and its expression level was measured by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The Monacolin K production in transformants was measured by high performance liquid chromatography (HPLC). The morphological features of mycelia and spore of the wild-type strain and the transformants were observed by scanning electron microscope (SEM). The results showed that 240 transformants were obtained by hygromycin resistance selection. Nine of these transformants were selected for the measurement of mok E expression by RT-qPCR.The expression levels of mok E gene were enhanced in three transformants, T2, T8 and T9. The production rates of Monacolin K lactone in transformants T2, T8 and T9 were 2 159.7, 4 177.6, and 3 365.7 μg/g, respectively, which were increased by 49.2%, 188.5% and 132.5%, respectively, compared with that in the wild-type strain (1 447.8 μg/g). The results of SEM revealed that mok E overexpression contributed to the morphological changes of mycelia and spore.关键词
红曲霉/基因过表达/Monacolin K/逆转录实时荧光定量聚合酶链式反应/扫描电镜/孢子Key words
Monascus spp./overexpression/Monacolin K/reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR)/scanning electron microscope (SEM)/spore分类
化学化工引用本文复制引用
林琳,王昌禄,李贞景,陈勉华,武淑芬,任志远..mok E基因过表达对红曲霉Monacolin K产量、菌丝及孢子形态的影响[J].食品科学,2018,39(8):45-49,5.基金项目
国家自然科学基金面上项目(31330059) （31330059）
