食品科学2018，Vol.39Issue(8)：50-56,7.DOI:10.7506/spkx1002-6630-201808009
响应面试验优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸工艺
Optimization of Succinic Acid Production from Crude Glycerol by Actinobacillus succinogenes GXAS137 Using Response Surface Methodology
摘要
Abstract
The optimization of medium components for improved succinic acid production by Actinobacillus succinogenes GXAS137 at reduced cost was performed using one-factor-at-a-time method and response surface methodology. The independent variables considered were electron acceptors, crude glycerol concentration and nitrogen sources. The results showed that dimethyl sulfoxide (DMSO) was the optimum electron acceptor and corn steep liquor (CSL) could be used as an alternative nitrogen source to replace yeast extract. The optimum culture medium that provided maximum succinic acid yield of 37.02 g/L, which was increased by 119% compared to that (16.88 g/L) before optimization, was composed of crude glycerol 55.43 g/L, DMSO 10.35 g/L and CSL 17.69 g/L. Under this optimized condition, the conversion rate of glycerol to succinic acid was 66.79% and succinic acid productivity was 0.51 g/(L·h). This study can provide a basis for the microbial production of succinic acid from crude glycerol.关键词
产琥珀酸放线杆菌GXAS137/粗甘油/丁二酸/单因素试验/响应面试验Key words
Actinobacillus succinogenes GXAS137/crude glycerol/succinic acid/one-factor-at-a-time experiments/response surface methodology分类
生物科学引用本文复制引用
张红岩,朱婧,冯英,李亿,秦艳,王青艳,梁戈,申乃坤..响应面试验优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸工艺[J].食品科学,2018,39(8):50-56,7.基金项目
国家自然科学基金地区科学基金项目(31660022) （31660022）
广西自然科学基金项目(2016GXNSFBA380205) （2016GXNSFBA380205）
南宁市科学研究与技术开发计划项目(20071004-1) （20071004-1）
广西民族大学相思湖青年学者创新团队项目(No.201706) （No.201706）
