食品科学2018，Vol.39Issue(8)：77-82,6.DOI:10.7506/spkx1002-6630-201808013
特基拉芽孢杆菌精氨酸酶基因的重组表达及酶学性质
Expression in Escherichia coli and Characterization of Arginase from Bacillus tequilensis
摘要
Abstract
Arginase is an alkaline hydrolases that catalyzes the production of urea and L-ornithine from arginine. An arginase gene encoding 297 amino acids was cloned from Bacillus tequilensis PanD37.By recombinant DNA technique,a genetically engineered strain of Escherichia coli was constructed for heterologous expression of the gene.Enzymatic assays indicated that the recombinant enzyme exhibited maximum activity at pH 10.0 and 45 ℃. Its activity was stable in the temperature range of 50-60 ℃ and in the pH range of 8.0-10.0. The enzymatic activity could be significantly activated by Mn2+, Ni2+, and Co2+. The activity of crude enzyme was 1 109.8 U/mL.关键词
精氨酸酶/特基拉芽孢杆菌/L-鸟氨酸/酶学特性Key words
L-arginase/Bacillus tequilensis/L-ornithine/enzymatic properties分类
生物科学引用本文复制引用
赵齐,冯志彬,秦雪,徐勤省,任梦云,潘潇,张洪霞,张娟..特基拉芽孢杆菌精氨酸酶基因的重组表达及酶学性质[J].食品科学,2018,39(8):77-82,6.基金项目
农业部转基因生物新品种培育重大专项(2016ZX08004002) （2016ZX08004002）
烟台市科技计划项目(2015ZH071) （2015ZH071）
