食品科学2018，Vol.39Issue(8)：129-134,6.DOI:10.7506/spkx1002-6630-201808021
不同泌乳期人乳与牛乳中游离氨基酸的对比
Comparison of Free Amino Acids between Human and Bovine Milk at Different Stages of Lactation
摘要
Abstract
This study aimed to detect and compare the free amino acid composition in human and bovine milk at different lactation stages using isobaric tags for relative and absolute quantification (iTRAQ) combined with high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) (HPLC-MS/MS-iTRAQ). The results indicated that human milk contained more types and higher contents of free amino acids compared with bovine milk. In addition, the total content of free amino acids showed a declining trend during the whole lactation period. The total contents of free amino acids were 0.32, 0.16, 0.63 and 0.37 g/L in bovine colostrum, bovine milk, human colostrum and human milk, respectively. Among 42 free amino acids detected in this study, 35 were found in human milk and 31 in bovine milk. Moreover, the contents of 25 free amino acids in human milk were higher than in bovine milk. In particular, a significant increase in histidine, phenylalanine and threonine were observed (P < 0.05). The content of free glutamic acid was the highest in bovine milk, human colostrum and human milk, while taurine was the most abundant free amino acid in bovine colostrum. Understanding the differences in free amino acid composition between human milk and bovine milk can lay a theoretical foundation for detailed studies on amino acid functions and metabolomics in human milk. In addition, this study can provide a basis for producing infant milk powder and functional dairy products.关键词
牛乳/人乳/同位素标记相对和绝对定量技术/游离氨基酸Key words
milk/human milk/iTRAQ/free amino acid分类
轻工纺织引用本文复制引用
吴尚仪,岳喜庆,吴尚,韩宏娇,孔繁华,关博元,张熙桐,曹雪妍,康世墨,陶冬冰..不同泌乳期人乳与牛乳中游离氨基酸的对比[J].食品科学,2018,39(8):129-134,6.基金项目
"十二五"农村领域国家科技计划项目(2013BAD18B03-02) （2013BAD18B03-02）
