食品科学2018，Vol.39Issue(8)：141-148,8.DOI:10.7506/spkx1002-6630-201808023
海南无刺蜂蜂蜜中多酚类物质成分分析及其抗氧化、抗炎活性评价
Polyphenic Constituents and Antioxidant and Anti-Inflammatory Activities of Stingless Bee Honey from Hainan
摘要
Abstract
This study aimed to analyze the physicochemical properties and polyphenolic composition and to evaluate the antioxidant and anti-inflammatory activities of stingless bee honey (SBH) from Hainan, China. The polyphenolic compounds were analyzed by liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). The contents of total phenolics and total flavonoids in SBH extract were determined by the Folin-Ciocalteu method and AlNO3colorimetry, respectively.The antioxidant activity of SBH was assayed in vitro by using 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate radical) (ABTS+·) scavenging and reducing power methods. Its anti-inflammatory effect was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results showed that SBH possessed the physicochemical properties: moisture content (26.3 ± 0.1)%, pH 3.7 ± 0.2, protein content (628.2 ± 9.0) mg/kg, amylase activity (19.6 ± 0.2) mL/(g·h), fructose content (21.0 ± 3.7)%, glucose content (24.9 ± 2.2)%, sucrose content (0.8 ± 0.1)%. The contents of total phenolics and total flavonoids in the extract were (96.6 ± 0.4) μg CAE/g and (16.1 ± 0.3) μg QE/g, respectively. The IC50values of the extract for scavenging of DPPH and ABTS radicals were (435.1 ± 0.4) and (423.0 ± 0.3) μg/mL, respectively, and its ferric reducing power was (0.6 ± 0.02) μmol Trolox/g. SBH exhibited potent concentration-dependent anti-inflammatory effects by inhibiting nitric oxide (NO) release from LPS-stimulated RAW 264.7 cells,markedly decreasing the mRNA expression of iNOS,IL-1β,IL-6 and MCP-1,and significantly increasing the mRNA expression of HO-1.These results suggest that SBH was rich in nutrients,phenolic acids and flavonoids,and had potent antioxidant and anti-inflammatory properties, making it very promising.关键词
无刺蜂蜂蜜/成分分析/抗炎/抗氧化Key words
stingless bee honey/food composition analysis/antioxidant/anti-inflammatory分类
农业科技引用本文复制引用
梁馨文,李强强,高景林,王凯,吴黎明..海南无刺蜂蜂蜜中多酚类物质成分分析及其抗氧化、抗炎活性评价[J].食品科学,2018,39(8):141-148,8.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-IAR) （CAAS-ASTIP-2016-IAR）
国家现代农业(蜂)产业技术体系建设专项(CARS-45) （蜂）
