食品科学2018，Vol.39Issue(8)：224-230,7.DOI:10.7506/spkx1002-6630-201808035
基于纳米金的过氧化物模拟酶比色检测乐果
Colorimetric Detection of Rogor Using Gold Nanoparticles as Peroxidase Mimetics
摘要
Abstract
This study developed a simple, rapid and visual approach for colorimetric detection of organophosphorus pesticide. Positively-charged gold nanoparticles (AuNPs) with an average diameter of 25 nm were synthesized by seed growth method which possessed intrinsic peroxidase-like activity. AuNPs were demonstrated to have the highest oxidase activity at pH 4.0, 35 ℃ in the presence of 1 mmol/L 3,3',5,5'-tetramethylbenzidine (TMB) and 10 mmol/L H2O2. It was shown that the nanozyme activity of AuNPs could be inhibited by its surface passivation with rogor, and so an inverse relationship between AuNPs catalytic activity and rogor concentration in the range from 1 to 80 mmol/L was found. The correlation coefficients and sensitivity of the assay were 0.985 and 1.39 mmol/L, respectively. Recoveries from tea samples spiked with different concentrations of rogor were in the range of 91.36%-102.56%. The effective peroxidase-like activity of AuNPs was kept for 1 month at room temperature. Adsorption of rogor onto AuNPs via amide bond was confirmed by Fourier transform infrared (FTIR) spectroscopic analysis. This assay may have potential application in environmental pollutant detection.关键词
纳米金/过氧化物模拟酶/有机磷农药检测/比色分析法Key words
gold nanoparticles/peroxidase mimetics/organophosphorus pesticides/colorimetric analysis分类
轻工纺织引用本文复制引用
邹小波,石海军,黄晓玮,石吉勇,翟晓东,蒋彩萍,李志华..基于纳米金的过氧化物模拟酶比色检测乐果[J].食品科学,2018,39(8):224-230,7.基金项目
国家自然科学基金面上项目(31671844 （）
31601543) （）
"十二五"国家科技支撑计划项目(2015BAD17B04) （2015BAD17B04）
"十三五"国家重点研发计划重点专项(2016YFD0401104) （2016YFD0401104）
江苏省自然科学基金项目(BK20160506) （BK20160506）
中国博士后科学基金项目(2016M590422 （）
2017T100334) （）
