食品科学2018，Vol.39Issue(8)：267-275,9.DOI:10.7506/spkx1002-6630-201808042
QuEChERS-超高效液相色谱-串联质谱法同时测定蔬果中34种植物生长调节剂的残留量
Determination of 34 Plant Growth Regulator Residues in Vegetables and Fruits by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for the determination of 34 plant growth regulators in vegetables and fruits was established. Samples were pretreated by the quick, easy, cheap, effective, rugged and safe (QuEChERS) method after being ground. Five grams of the samples were extracted with 10 mL of acetonitrile, and dehydrated with a mixture of 4 g of MgSO4, 1 g of NaCl, 1 g of Na3Cit, 0.5 g of 2Na2HCit·3H2O. The supernatant extract was purified with different sorbents according to the state of samples. The analytes were separated on a Waters Acquity HSS T3 column with acetonitrile-water as the mobile phase using gradient elution, detected by mass spectrometry using electrospray ionization with piecewise scanning in the multiple-reaction monitoring (MRM) mode and quantified by the matrix-matched external standard peak area method. The calibration curves were linear in the concentration range of 2-100 μg/kg. The limits of detection (LODs) of 34 plant growth regulators were 2-10 μg/kg. The method was rapid, sensitive and precise, and could meet the requirements for the determination of plant growth regulator residues thereby providing technical support for regulation of plant growth regulator residues in common vegetables and fruits.关键词
QuEChERS/超高效液相色谱-串联质谱法/植物生长调节剂/残留检测Key words
QuEChERS/liquid chromatography-tandem mass spectrometry/plant growth regulators/residue determination分类
化学化工引用本文复制引用
郝杰,姜洁,毛婷,孙晓冬,杨丽梅,张朝晖,崔凤云..QuEChERS-超高效液相色谱-串联质谱法同时测定蔬果中34种植物生长调节剂的残留量[J].食品科学,2018,39(8):267-275,9.基金项目
北京市科技计划项目(D151100003815002) （D151100003815002）
