食品与发酵工业2018，Vol.44Issue(3)：15-21,7.DOI:10.13995/j.cnki.11-1802/ts.015964
酵母源葡萄糖耐量因子GTF的纯化及其铬价态分析
Study on purification of glucose tolerance factor and its chromium valence state
摘要
Abstract
Glucose tolerance factor (GTF) can effectively regulate sugar metabolism,improve the affinity of insulin to receptor,and improve fat metabolism.Up to now,its chromic value in GTF is unclear,which limited its application in reducing sugar health food.In this paper,extraction of GTF with ammonia and ethanol method from high chromium yeast was optimized.And then glucose tolerance factor (GTF) with high purity was obtained by two stages of gel chromatography.HPLC-ICP-MS was used to analyze chromium valence of GTF.The high chromium yeast was treated with 0.1 mol/L of ammonium for 4 h and then 30％ of ethanol for 20 min.The total chromium in this extraction was 133 μg.Compared with ammonia extraction alone,the ratio of chromium to protein was increased by 6 times in this way.After extraction,glucose tolerance factor was purified by tow chromatography consecutively.The purified GTF was analyzed by HPLC-ICP-MS.Its chromatography showed that there was only one peak of chromium in this sample.It appeared in range from 31 s to 90 s,which was in agreement with standard of trivalent chromium.There-fore,it could be concluded that chromium in GTF exist in the state of trivalent.This will provide basis for research and development of high chromium yeast for dietary supplement.关键词
葡萄糖耐量因子/乙醇提取/高效液相色谱/电感耦合等离子体质谱/价态分析/Cr(Ⅲ)Key words
glucose tolerance factor (GTF)/ethanol extract/high performance liquid chromatography (HPLC)/inductively coupled plasma mass spectrometry (ICP-MS)/valence state analysis/chromium (Ⅲ)引用本文复制引用
许乐,李函彤,逄晓阳,张书文,芦晶,吕加平,刘鹭..酵母源葡萄糖耐量因子GTF的纯化及其铬价态分析[J].食品与发酵工业,2018,44(3):15-21,7.基金项目
国家自然基金面上项目(No.31371763) （No.31371763）
中央级公益性科研院所基本科研业务费(S2016JC01)专项资助 （S2016JC01）
