食品与发酵工业2018，Vol.44Issue(3)：179-185,7.DOI:10.13995/j.cnki.11-1802/ts.014443
有机硒营养液对蓝莓营养品质和抗氧化作用的影响
Effect of organic selenium solution on the nutrition quality and antioxidant function of blueberry
摘要
Abstract
The effect of selenium nutrient solution were studied on nutrition quality and antioxidant function of blueberry,so as to provide evidence in developing new natural function food.Solution of selenium nutrient was sprayed on the leaves of blueberry at the late stage of fruit setting and the fruit formation.The fruit nutrition qualities of blueberry,such as nutrient composition,function elements and superoxide dismutase (SOD) activity were analyzed by spectrophotometric method,kjeldah method,atomic fluorescence photometer method and QRS method.In order to study the effects of organic selenium blueberry on ROS in cells,the HepG2 cell were pretreated with organic selenium blueberry before exposure to DCFH-DA,and then the variation of ROS was detected using laser scanning confocal microscope.For the antioxidant level experiment of organic selenium blueberry in vivo,the mouse was fed with organic selenium blueberry for one month.Then the blood and liver of mice were collected for analysis.The results showed that compared with the water and sodium selenite groups,the content of protein,soluble sugar,vitamin C,total phenolics,flavone,selenium,and the activity of SOD raised significantly treated with selenium solution.The content of vitamin E and anthocyanidin sprayed selenium nutrient solution was also improved in a certain degree.The increase of ROS was obviously inhibited by organic selenium blueberry.Comparing to control group,When the mouse were feeded with organic selenium blueberry or selenium blueberry,the content of MDA was obviously reduced to 51.53 ％ or 29.81％,the activity of SOD was obviously increased to 45.93％ and 29.62％,and the activity GSH-Px was increased to 55.94％ or 38.02％.The influence of antioxidant enzymes by organic selenium blueberry was greater.Our results indicated that organic selenium blueberry has a significant effect against oxidative damage by nutrition elements optimization and immune function enhancement.关键词
蓝莓/有机硒/营养品质/抗氧化能力Key words
blueberry/organic selenium/nutrition quality/antioxidant capacity引用本文复制引用
李春霞,刘伟,陈凌云,姜文仪,蒋光月,黄蓓..有机硒营养液对蓝莓营养品质和抗氧化作用的影响[J].食品与发酵工业,2018,44(3):179-185,7.基金项目
安徽省科技攻关项目(1301032140 （）
1501041177) （）
