中国食品添加剂Issue(3)：80-87,8.
大孔吸附树脂分离纯化翻白草中三萜类化合物及其降血糖作用的研究
Purification of triterpenoids from Potentilla discolor by macroporous resin and the anti-diabetic effects
摘要
Abstract
To study the isolation and purification technology parameters of oleanlic acid and total triterpenoids from Potentilla discolor by macroporous resins.Eight macroporous resins were selected with static and dynamic adsorption and desorption assays,and we found that AB-8 macroporous resin was the optimal purification resin.The further orthogonal design was used to study the effects of macroporous resins purification technology with the oleanlic acid and total triterpenoids were served as indexs.And the hypoglycemic effect of triterpenoids was evaluated on diabetes model mouse.In conclusion,the results indicated that the optimal purification conditions were sample mass concentration of 250 g/L,the loaded amount of 3 BV,and the flow rate of 2 BV/h.The collection was obtained by 30％ ethanol with 4BV and 85％ ethanol of 7BV,respectively.The purity of oleanlic acid and total triterpenoids were 2.67％ and 58.46％ after the isolation and purification processes.The results indicated that the AB-8 macroporous resin was an ideal resin with the best enrichment for isolating total triterpenoids from Potentilla discolor and the method obtained by orthogonal design was easy to conduct.In addition,the triterpenoids from Potentilla discolor possess anti-diabetic effects of diabetic mice.关键词
大孔吸附树脂/翻白草/齐敦果酸/总三萜/降血糖Key words
Macroporous resins/Potentilla discolor/oleanlic acid/total triterpenoids/hypoglycemic分类
医药卫生引用本文复制引用
邵长森,韩真真,张国青,林桂涛,曲远均..大孔吸附树脂分离纯化翻白草中三萜类化合物及其降血糖作用的研究[J].中国食品添加剂,2018,(3):80-87,8.基金项目
山东省中医药科技发展计划项目(编号:2013-059). （编号:2013-059）
